graphpad prism 6 two sets of symbols one graph

Thus NPY-induced changes in Y receptor motility and brightness reflect early events surrounding arrestin dependent endocytosis at the plasma membrane, results supported by a novel combined BiFC/FCS approach to detect the underlying receptor-β-arrestin signalling complex. For example, if you have a y axis in 100 increments and x axis in 1 increments, your curve will look a bit distorted.

The importance of β-arrestin recruitment for these effects was illustrated by reduced lateral mobility ( D 1.20–1.33 × 10 − 9 cm 2 s − 1) of Y1 receptor-β-arrestin BiFC complexes. One limitation of the package, however, is that the dimensions of the bracket are set by the dimensions of the x and y axis. NPY treatment also increased Y1 receptor-GFP particle brightness, changes which indicated receptor clustering, and which were abolished by the 6A mutation. Agonist induced changes in Y1 receptor motility were inhibited by mutations (6A) which prevented β-arrestin recruitment and internalisation conversely they became apparent in a Y2 receptor mutant with increased β-arrestin affinity. At a concentration which promoted only Y1 receptor endocytosis, NPY treatment reduced Y1-GFP motility ( D 1.48 × 10 − 9 cm 2 s − 1), but did not alter diffusion characteristics of the Y2-GFP receptor. Diffusion co-efficients ( D) for Y1 and Y2-GFP receptors in HEK293 cell plasma membranes were 2.22 and 2.15 × 10 − 9 cm 2 s − 1 respectively. Double-click on the X axis to bring up Format Axis.

This video is part of the Essential Statistics series, presented by Dr James Clark, from the School of Cardiovascular Medicine and Sciences at King’s College London. Understand the results of descriptive and normality tests. A novel GFP-based bimolecular fluorescence complementation (BiFC) system also identified Y1 receptor-β-arrestin complexes. To make the graph horizontal (as below), double click on the graph to bring up Format Graph, and go to the third tab. Use Prism to perform statistics to describe your data sets. We investigated the influence of β-arrestin adaptors and endocytosis mechanisms on plasma membrane diffusion and particle brightness of GFP-tagged neuropeptide Y (NPY) receptors. A tutorial describing the steps required to perform correlation analysis in GraphPad PrismAll footage is the property of James Clark©2019 Dory Video/James Cl. However, relating these properties to molecular mechanisms can be challenging.

Fluorescence correlation spectroscopy (FCS) and photon counting histogram (PCH) analysis are powerful ways to study mobility and stoichiometry of G protein coupled receptor complexes, within microdomains of single living cells.

Graphpad prism 6 two sets of symbols one graph